Journal: CNS Neuroscience & Therapeutics

Article Title: Electroacupuncture Prevents Against AD‐Like Phenotypes in APP/PS1 Mice: Investigation of the Mechanisms From Cerebral Microangiopathy

doi: 10.1002/cns.70696

Figure Lengend Snippet: Electroacupuncture inhibits abnormal expression of microvessels and inflammation‐related proteins in APP/PS1 mice. (A) Representative immunoblots of PDGFRβ and CD31 expression in the prefrontal cortex. (B) Representative immunoblots of PDGFRβ, CD31, and GLUT1 expression in the hippocampus. (C) Representative immunoblots of Occludin, Claudin‐5, ZO‐1, NF‐κB, TNF‐α, and IL‐1β expression in the hippocampus. (D) Quantitative data of PDGFRβ and CD31 expression in the prefrontal cortex. (E) Quantitative data of PDGFRβ, CD31, and GLUT1 expression in the hippocampus. (F) Quantitative analysis of Occludin, Claudin‐5, and ZO‐1 expression in the hippocampus. (G) Quantitative analysis of NF‐κB, TNF‐α, and IL‐1β expression in the hippocampus. Data are expressed as mean ± SEM ( n = 3 per group). * p < 0.05 between groups. (one‐way ANOVA followed by Tukey test).

Article Snippet: After blocking with 5% non‐fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies overnight at 4°C: GAPDH (1:1000, BM3874, Boster, China), platelet‐derived growth factor receptor beta (PDGFRβ) (1:1000, A00096‐1, Boster, China), Zonula occludens‐1 (ZO‐1) (1:1000, PB9234, Boster, China), Claudin 5 (1:1000, 29,767–1‐AP, Proteintech, China), Occludin (1:1000, A01246‐4, Boster, China), NF‐κB (1:1000, CY5034, Abways, China), TNF‐α (1:1000, RM8040, Biodragon, China), IL‐1β (1:1000, CY5087, Abways, China), BDNF (1:1000, E17G19, Selleck, China), PSD95 (1:1000, F17C8, Selleck, China), CD31 (1:1000, 11,265–1‐AP, Proteintech, China), glucose transporters 1 (GLUT1) (1:1000, P22E15, Selleck, China).

Techniques: Expressing, Western Blot